laboratory assistant ii interview questions shared by candidates
Explain step by step of how you do blood culture?
If you are prepare, you should know the answer!!
Blood Culture; Microbial test to find out bacteremia or septicaemia .it is done by culturing blood in thyoglycolate borth for upto 5 days of continuous evaluation for any bacterial growth Method of blood collection Venipuncture made at anticubetal fossa of the right or left hand.Before this clean the area with 70% isopropyl alcohol Procedure 10 ml of blood is inoculated in two or more bottles of thyoglycolate broth and incubated anaerobically and aerobicaly at 37degree Celcious(room Temperature) for upto 5 cosecutive days.Check each day for bacterial growth.Repot negative oonly after the 5th day Result if positive roprt to physician as bacteremia do gram stain for identificaion of the organism culture should be be done with slective media for 3 days and also do antsensitivity test in order to isolate the bacteria